Journal:

Article Title: Clustering and Redistribution of Late Endocytic Compartments in Response to Helicobacter pylori Vacuolating Toxin

doi: 10.1091/mbc.E03-08-0618

Figure Lengend Snippet: Redistribution of late endocytic markers in response to VacA. (A-D) HeLa cells were transiently transfected with pEGFP-Rab7 (A and B) or pEGFP-Rab5 (C and D). Twenty-four hours after transfection, cells were either treated with 5 μg/ml acid-activated VacA in the absence of supplemental ammonium chloride (B and D) or left untreated (A and C) for 20 h. (E and F) AGS cells were transiently transfected with pEGFP-Rab7 and then treated with VacA (F) or left untreated (E). (G-N) Nontransfected HeLa cells were treated with 5 μg/ml acid-activated VacA (H, J, L, and N) or left untreated (G, I, K, and M). Twenty hours later cells were fixed, permeabilized, and stained with antibodies to LAMP-1 (G and H), cathepsin D (I and J), Rab9 (K and L), or EEA1 (M and N). VacA induced redistribution of EGFP-Rab7, LAMP-1, and cathepsin D from a scattered distribution to perinuclear aggregates, but it did not affect the localization of EGFP-Rab5, Rab9, or EEA1. Bar, 10 μm.

Article Snippet: The rabbit anti-cathepsin D polyclonal antibody was from Biomeda (Foster City, CA).

Techniques: Transfection, Staining